Some success in interferon production by bacteria has recently been achieved by the recombinant dna technique . 近年曾經(jīng)應(yīng)用重組DNA技術(shù)使細(xì)菌產(chǎn)生干擾素獲得了一定成效。
Recombinant dna techniques deeloped for cloning genes 重組dna技術(shù)被應(yīng)用于基因克隆
Recombinant dna techniques developed for cloning genes 重組dna技術(shù)被應(yīng)用于基因克隆
Some success in interferon production by bacteria has recently been achieved by the recombinant dna technique 近年曾經(jīng)應(yīng)用重組dna技術(shù)使細(xì)菌產(chǎn)生干擾素獲得了一定成效。
In the work , we got mutated plasmids pgex - 6p - m - centrein by means of pcr and recombinant dna techniques 隨著生物技術(shù)的逐步成熟,定點突變成為了科研人員進(jìn)行科學(xué)研究的有力手段。
By recombinant dna techniques , the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb . the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e . coli dh5a and induced with iptg . sds - page analysis showed an induced expression product band about 72ku , which correspond to the sizes of vp2 , reported in the literature 利用dna重組技術(shù),將其結(jié)構(gòu)蛋白vp2基因亞克隆至原核表達(dá)載體pproex - htb , iptg誘導(dǎo)后成功表達(dá)出與預(yù)期大小相符的約72ku的融合蛋白,光密度掃描對表達(dá)產(chǎn)物進(jìn)行初步定量,表明表達(dá)產(chǎn)物約占菌體總蛋白的14 。